Understanding living systems through the lens of physical principles is a cornerstone objective of biophysics. Historically, physicists have delved into this challenge by exploring a myriad of biological phenomena. As a consequence, a rich collection of theoretical methodologies has been developed. While these techniques often have their origins in other physics domains, they also have evolved in distinct ways in biology. In this talk, we demonstrate how theoretical methods, being common in physics, can be used to analyze biological phenomena, such as evolutionary processes and intracellular reactions. We place specific emphasis on the techniques of path integral method and thermodynamic structure, highlighting that these aren’t merely computational tools but that they enable us to elucidate fundamental aspects of living phenomena.

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